Bioreactors can be categorized into various types according to different classification perspectives. According to the categorization of the type of design working principle, there are several different bioreactors.
1. Stirred Bioreactor: This is the most common and versatile type, where mixing of the culture medium and oxygen transfer are realized by mechanical stirrer. The advantages are simple process, flexible operation, easy to scale up, suitable for mass production, and can provide a homogeneous culture environment to ensure stable product quality. The disadvantage is that high shear force may cause damage to some sensitive cells. It is widely used in microbial fermentation (food, amino acids, antibiotics, etc.) and mammalian cell suspension culture (therapeutic antibodies, viral vaccines, etc.).
2. Airlift Bioreactor: Utilizes the rising motion of gas bubbles to mix the culture medium. It has low shear force and is suitable for cells that are sensitive to shear force. The advantage is low shear, but mixing efficiency and oxygen transfer may not be as efficient as in stirred reactors.
3. Perfusion Bioreactor: A continuous or semi-continuous culture that maintains cell growth and metabolite accumulation by constantly replenishing fresh medium and removing portions of the culture solution. It allows for high cell density culture and increased productivity, but with higher operational complexity. Commonly used for mammalian cell culture.
4. Fixed-bed Bioreactor: Cells or microorganisms are immobilized on a carrier and the culture medium flows through the carrier. Advantages are low shear, easy to continuous culture and cell retention, but there are problems such as insufficient mixing and oxygen supply, easy to clog. Suitable for some specific microbial culture or enzyme production.
5. Hollow Fiber Bioreactor: Cells are grown inside the hollow fiber membrane and the medium flows outside the membrane. The advantages are mild culture environment, high cell density, easy to purify, but the scale is limited, not suitable for large-scale production. Commonly used for small-scale animal cell culture.
6. Single-use Bioreactor: The use of disposable plastic containers avoids the need for cleaning and sterilization processes, shortens the production cycle, and reduces the risk of cross-contamination. However, the scale is usually limited (generally no more than 2000L) and the oxygen transfer efficiency is relatively low.
Our bioreactor is stirred bioreactor which is flexible in operation and has good applicability. The culture process is easy to amplify, which can provide a homogeneous environment for the growth and proliferation of organisms. Product quality is stable and very suitable for industrialized production. The piping arrangement is relatively simple, which can provide better aseptic conditions and is not easy to be contaminated during cell growth
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